What are Stem Cells?
A mesenchymal stem cell is a primitive cell with the ability to:
Cell
cell
What are Stem Cells?
A mesenchymal stem cell is a primitive cell with the ability to:
  • Self-Replicate
  • Differentiate into
    multiple tissues
  • Bone
  • Cartilage
  • Muscle
  • Fat
  • Fight Apoptosis
    (Cell Death)
  • Reduce Inflammation

2012- Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells–a comparative study. – Institute of Veterinary Anatomy, Histology and Embryology

Abstract In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrowderived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent MSCs in small animal practice are joint diseases as MSCs of both sources have shown to possess chondrogenic differentiation ability. However, it is not clear whether the chondrogenic differentiation potential of cells of these two distinct tissues is truly equal. Therefore, we compared MSCs of both origins in this study in terms of their chondrogenic differentiation ability and suitability for clinical application. BMMSCs harvested from the femoral neck and ADSCs from intra-abdominal fat tissue were examined for their morphology, population doubling time (PDT) and CD90 surface antigen expression. RT-PCR served to assess expression of pluripotency marker Oct4 and early differentiation marker genes. Chondrogenic differentiation ability was compared and validated using histochemistry, transmission electron microscopy (TEM) and quantitative RT-PCR. Both cell populations presented a highly similar morphology and marker expression in an undifferentiated stage except that freshly isolated ADSCs demonstrated a significantly faster PDT than BM-MSCs. In contrast, BM-MSCs revealed a morphological superior cartilage formation by the production of a more abundant and structured hyaline matrix and higher expression of lineage specific genes under the applied standard differentiation protocol. However, further investigations are necessary in order to find out if chondrogenic differentiation can be improved in canine ADSCs using different protocols and/or supplements.

Keywords Dog . Mesenchymal stem cells . Chondrogenic differentiation . Cartilage . Osteoarthritis

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  • American Academy of Orthopaedic Surgeons
  •  American Orthopaedic Society for Sports Medicine (AOSSM)
  • International Society for Hip Arthroscopy
  • Arthroscopy Association of North America – AANA
  • RYC Orthopaedics
  • Pro Medical New York
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